BRCA1 plays a role in cell-cell contacts and invasion capacity
By growing cells to a near confluent monolayer, scraping a wound in the monolayer, and following the healing of the wound by time-lapse microscopy, it is possible to assess BRCA1 function in modulating the influence of cell-cell contact and external stimuli on cell motility.
Time-lapse videomicroscopy of CHO cells expressing eGFP-BRCA11634-1863, which show loss of cell–cell contacts and enhanced invasion capacity. Orange tracks show the enhanced invasion capacity of single cells; pink tracks point at loss of cell-cell contact and detachment of cells that migrate away from the front line into the wound. Playback speed, 12 FPS.