Movies: bloodstream-form tomography

Home Research Team Cat's CV Publications Movies Resources News Opportunities

Cytoarchitecture of African trypanosomes

These movies provide the supplementary information for the paper Gadelha et al. (2009) PNAS 106:17425-17430.
Supplementary Movie 1
3D electron tomographic reconstruction of the anterior flagellar pocket region and surrounding organelles from a late M-phase bloodstream-form trypanosome. This reconstruction is derived from a 3 x 1 montaged, three-serial section tomogram yielding a total of 457 tomographic slices.
thumb
Supplementary Movie 2
3D electron tomographic reconstruction seen in Supplementary Movie 1 merged with the resultant 3D segmentation model (which can be seen in Supplementary Movie 3).
thumb
Supplementary Movie 3
A segmentation model of the organelles in close proximity to the flagellar pocket. The model was generated from an electron tomographic reconstruction of a bloodstream form African trypanosome cell. The boundaries of individual organelles have been traced so that their relationship can be investigated in 3-dimensions.
thumb