Movies: mammalian cell motility

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A novel role for BRCA1 in regulating breast cancer cell spreading and motility

These movies provide the supplementary information for the paper Coene et al. (2011) JCB 192:497-512.
Supplementary Movie 1
BRCA1 is recruited to plasma membrane ruffles and plays a role in cell motility. This video shows the dynamic behaviour of membrane ruffles in CHO cells that strongly express eGFP-BRCA11634-1863. Time-lapse videomicroscopy over 4 hours; playback speed: 7 FPS.
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Supplementary Movie 2
BRCA1 plays a role in cell-cell contacts and invasion capacity. Time-lapse videomicroscopy of CHO cells expressing eGFP-BRCA11634-1863, which show loss of cell–cell contacts and enhanced invasion capacity. Orange tracks show the enhanced invasion capacity of single cells; pink tracks point at loss of cell-cell contact and detachment of cells that migrate away from the front line into the wound. Playback speed, 12 FPS.
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Supplementary Movie 3
BRCA1 plays a role in cell-cell contacts and invasion capacity. Time-lapse videomicroscopy of CHO wild type cells (CHO-WT) vs. CHO cells expressing high levels of eGFP-BRCA11634-1863 (CHO-High). Loss of cell-cell contact and independent migration of separated cells into the wound space was most apparent in CHO-High cells. As a result, the perimeter of wounds in CHO-High monolayers appeared discontinuous and irregular in contrast to the more uniform boundary of wounds in CHO-WT. Playback speed, 12 FPS.
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