VectorsWe have recently published a genetic toolkit specifically designed for the endogenous-locus tagging of membrane protein genes encoding GPI-anchored and non-GPI-anchored sequences containing N-terminal signal sequences, as described in Gadelha et al. (2015). I’m currently preparing maps and jpegs so to make plasmid sequences available here. Please check again soon.
ImageJ macrosThis section provides tools to aid in the analysis of flagellar ultrastructure and beating. The files are macros for the fantastic Open Source image manipulation and analysis software ImageJ. A huge amount of extra functionality is freely available for the program in the form of plugins. Macros are similar, but generally quicker to write and not quite as slick looking. You can do all of the tasks provided by these macros the long way around, but they should speed things up considerably.
These scripts are Open Source - cut, copy and modify to your heart's content, but an acknowledgement would be kind.